General Cell Based Assays
Eurofins Discoverx AssayComplete Cell Plating 13 Reagent, 500. (93-0563R13B)
AssayComplete Cell Plating 13 Reagent is an optimized cell plating reagent for use in plating of Eurofins DiscoverX cell lines for assays. This reagent was optimized for maximal cell health and ligand responsiveness during assays while eliminating hormones and growth components which may lead to increased basal activity. This product is provided as a bottle of 500 mL. Please refer to cell line specific datasheet to determine the recommended cell plating reagent. For detailed product information, User manual and datasheet, visit www.discoverx.com/reagents.
Eurofins Discoverx PathHunter Detection Kit, 550 (100 plates), 40,000 dp (384-well). (93-0001L)
The PathHunter Detection Kit is a detection kit intended for use with Eurofins DiscoverX's PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 100 plates (96/384 well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0001l.
Eurofins Discoverx cAMP Hunter eXpress MC3R CHO-K1 GPCR Assay, 2-Plate Kit. (95-0045E2CP2M)
The cAMP Hunter MC3R eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. α-melanocyte stimulating hormone) based activation of MC3R GPCR activity via detection of increasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type MC3R, a naturally Gs coupled GPCR. Upon activation of MC3R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical DuP-697 asy Reagent 1ea
An alkaloid with anti-inflammatory activity reduces LPS-induced TNF-a release from and NO production in RAW 264.7 macrophages at 10 30 and 100 UM reduces paw swelling in a rat model of CFA-induced arthritis at 9 and 27 mg/kg reduces colonic inflammatory cytokine levels and maintains mucosal barrier function in a mouse model of DSS-induced colitis from 25-100 mg/kg
Eurofins Discoverx PathHunter eXpress TrkB-P75 Functional Assay, 2-Plate Kit. (93-0530E3CP16M)
The PathHunter eXpress TrkB-P75 Functional Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting RTKs. This kit assesses RTK activation via detection of activated TrkB and P75 dimerization-induced SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses ProLink-tagged TrkB and an EA-tagged SH2 domain protein. It also overexpresses an untagged coreceptor, P75. Upon ligand-induced receptor activation, SH2-EA recruitment leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Adalimumab Bioassay Kit, 2-Plate. (93-0538B15-00131)
The PathHunter Adalimumab Bioassay Kit provides a robust, highly sensitive, and easy-to-use functional cell-based assay to measure drug potency and detect neutralizing antibodies. The bioassay kit contains all the materials needed to run the assay, including cryopreserved cells, cell plating reagent, control agonist, detection reagents, and assay plates. The ready-to-use cryopreserved cells have been manufactured for single-use to ensure assay reproducibility, and faster implementation from characterization to lot release. This assay has been optimized and qualified with Humira (not supplied in the bioassay kit). Humira is a registered trademark of AbbVie, Inc. For detailed product information, User manual and COA, visit www.discoverx.com/bioassay.
Eurofins Discoverx PathHunter eXpress CHRM4 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0349E2CP0M)
The PathHunter CHRM4 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. acetylcholine) based activation of CHRM4 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged CHRM4 and EA-tagged β-Arrestin-2. Activation of CHRM4 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Revvity Health Sciences Inc HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 50,000 Assay Points
HTRF Human & Mouse Phospho-CDK2 (Tyr15) Detection Kit, 50,000 Assay Points
Revvity Health Sciences Inc AlphaLISA Human IL-8 High Performance Detection Kit, 500 Assay Points
AlphaLISA Human IL-8 High Performance Detection Kit, 500 Assay Points
Cayman Chemical L-Lactate asy KIt 96Wel
Cayman’s L-Lactate Assay provides both a fluorescence and an absorbance-based method for detecting L-lactate in biological samples such as serum, plasma, blood, urine, and saliva. It can also be utilized to determine intracellular and extracellular lactate concentrations in cell culture samples. In the assay, LDH catalyzes the oxidation of lactate to pyruvate, along with the concomitant reduction of NAD+ to NADH. NADH reacts with the substrate to yield a final product that can be measured fluorometrically with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm, or colorimetrically at 535 nm.
Cayman Chemical CelBased asy NBD CholSte 500uL
A DYRK1A inhibitor (IC50 0.4 nM) selective for DYRK1A over DYRK2 Clk1 Clk2 Clk3 Cdk2 and GSK3B (IC50s 19 7.1 9.4 54 100 and 94 nM respectively) but does inhibit DYRK1B (IC50 2.7 nM) inhibits DYRK1A-induced increases in tau phosphorylation in a cell-based assay (IC50 590 nM)
Molecular Devices Calcium Assay Kit, QF, Express, 96/384/1536-well
The FLIPR Calcium 6-QF Express Kit from Molecular Devices is a fluorescence-based, homogeneous calcium indicator assay designed for high-throughput intracellular calcium flux screening. The QF formulation supports quench-sensitive assays and multiplexed workflows, and the Express Kit supplies reagent vials sufficient for multiple microplates in 96-, 384-, and 1536-well formats.
- Homogeneous fluorescence-based calcium flux assay for cell-based screening.
- QF formulation for quench-sensitive targets and multiplexing.
- Contains 2 vials of Component A and 2 vials of Component C.
- Assay buffer (Component B) is not included in the Express Kit.
- Sufficient reagent for up to 100 96- or 384-well plates, or 150 1536-well plates.
- Suited for GPCR, ion channel, and other calcium signaling assays in high-throughput screening.
Molecular Devices CatchPoint cAMP Assay Kit, Fluorescent, 384-Well, Explorer
The CatchPoint cAMP Fluorescent Assay Kit provides a sensitive, quantitative fluorescence-based competitive immunoassay for measuring cyclic AMP (cAMP) in high-throughput 384-well workflows. The Explorer kit includes ready-to-use plates and reagents optimized for low cAMP levels and automation-compatible assays.
- Quantitative competitive fluorescent immunoassay for cAMP
- 384-well black well/clear-bottom plates, pre-coated and pre-blocked
- Includes two 384-well assay plates and required reagents and buffers
- Stoplight Red substrate provides a stable fluorescent readout
- Flexible read times from about 10 minutes up to 24 hours after substrate addition
- Compatible with high-throughput screening and automation